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"茁彩生物&zcibio"试剂盒,成功发表SCI文章

发布时间:2018-10-26 15:29 |  点击次数:

Examination of carbohydrate and lipid metabolic changes
during Haematococcus pluvialis non-motile cell germination
using transcriptome analysis
Qianqian Li1,2,3 & Litao Zhang1,2,3 & Jianguo Liu1,2,3
Received: 10 March 2018 / Revised and accepted: 16 May 2018
# Springer Science+Business Media B.V., part of Springer Nature 2018
Abstract
During the complex Haematococcus pluvialis life cycle, germination from non-motile cells to motile cells is the key stage for cell
recovery from resting spores. Three phases can be recognized: first, repeated mitotic events; next, cytokinesis to form the zoospore;
and finally, a fast release of motile cells. After HiSeq 2000 sequencing of RNA collected at four time points during non-motile cell
germination, a total of 2202 differentially expressed genes (DEGs) were identified. In the expression profiles, there is a consistent
increase in the expression level of α-amylase and ACAA1 in the entire process, indicating starch and lipid mobilization. In phase 1,
two down-regulated genes (fructose-1, 6-bisphosphate aldolase (FBA) and pyruvate kinase (PK)) of glycolysis limited the
provision of acetyl-CoA for the TCA cycle, which could be compensated by fatty acid degradation (up-regulation of ACAA1)
in the glyoxysome. In phase 2, nine and eight up-regulated enzymes of carbohydrate (glycolysis, TCA cycle, and pentose
phosphate pathway) and lipid (fatty acid synthesis and degradation) metabolism, respectively, would increase the metabolic rate
and come into a balance between production and consumption of starch and lipid. Till phase 3, the expression of the vast majority
of carbohydrate metabolism-related DEGs remained high, while lipid metabolism did not. This suggested that the carbon flux
centered on carbohydrate metabolism in this phase. In addition, several isozymes of FBA, GAPDH, PK, and so on were separated
by SMART analysis and are postulated to serve different actions during H. pluvialis non-motile cell germination.
Keywords Haematococcus pluvialis . Non-motile cell . Motile cell . Germination . Metabolic change